an endogenous immune adjuvant released by necrotic cells for enhancement of dna vaccine potency
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abstract
background: improving vaccine potency in the induction of a strong cell-mediated cytotoxicity can enhance the efficacy of vaccines. necrotic cells and the supernatant of necrotic tumor cells are attractive adjuvants, on account of their ability to recruit antigen-presenting cells to the site of antigen synthesis as well as its ability to stimulate the maturation of dendritic cells. objective: to evaluate the utility of supernatant of necrotic tumor cells as a dna vaccine adjuvant in a murine model. method: the supernatant of el4 necrotic cells was co-administered with a dna vaccine expressing the glycoprotein b of herpes simplex virus-1 as an antigen model under the control of cytomegalovirus promoter. c57bl/6 mice were vaccinated three times at two weeks intervals with glycoprotein b dna vaccine and supernatant of necrotic el4 cells. five days after the last immunization, cell cytotoxicity, ifn-γ and il-4 were evaluated. results: the obtained data showed that the production of ifn-γ from the splenocytes after antigenic stimulation in the presence of the supernatant of necrotic el4 cells was significantly higher than the other groups (p
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Journal title:
iranian journal of immunologyجلد ۹، شماره ۴، صفحات ۲۱۵-۲۲۵
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